To further demonstrate that VE821 functions by inhibiting GRSF1, we treated GRSF1-deficient MHCC-97H and Hep3B cells with VE821 and found that VE821 could not further reduce GRSF1-deficient HCC cell proliferation, colony formation, migration (p<0.05; Fig. S6A-D) and tumor growth (p<0.05; Fig. S7) in the background of GRSF1 depletion. Here, GRSF1 is linked to hepatocellular carcinoma.