To evaluate these mechanisms, we conducted transient transfection of H460 cells (human lung cancer cell line without variants or amplification of ERBB family genes) and NIH3T3 cells (mouse non-cancer fibroblast cell line) with an empty vector (empty) or a vector containing ERBB2 wild type (WT) or one of four ERBB2 variants. Here, ERBB2 is linked to cancer.