To further explore the underlying molecular mechanisms in glioblastoma cells treatment with anlotinib, the expression of many signaling proteins (p-JAK2, STAT3, p-STAT3, VEGFA, cyclin A2, cyclin D1, HMGB1, and MMP2) were determined in all three cell types by western blotting. This evidence concerns the gene CCND1 and glioblastoma.