In order to further determine the effects of GPR34 on the growth and proliferation of LS174T cells, exogenous LysoPS (optimized, 20 μM, Fig. 3a) proved as a stimulation for the migration of colorectal cancer cells through GPR34 and PI3K/Akt pathway, was added into the complete DMEM media. The gene discussed is GPR34; the disease is colorectal cancer.