To understand the clinical significance of the POU2F1-ALDOA axis, we stratified 184 colon cancer patients into (1) POU2F1high/ALDOAhigh, (2) POU2F1high/ALDOAlow, (3) POU2F1low/ALDOAhigh, (4) POU2F1low/ALDOAlow groups, based on the levels of POU2F1 and ALDOA expression in their colon cancer tissues. Here, ALDOA is linked to malignant colon neoplasm.