Interestingly, we found that POU2F1 and ALDOA expression were paralleled in colon cancer tissues and cell lines; altered POU2F1 expression modulated ALDOA expression in colon cancer cells; both POU2F1 and ALDOA expression appeared to a better biomarker for prognosis of colon cancer. The gene discussed is POU2F1; the disease is malignant colon neoplasm.