Among the 27 different mutations identified in 9 genes, 11 were novel, since they were not reported in deafness and Leiden Open Variation Database (LOVD) databases or in PubMed as related to pathology: c.12829C > T, p.(Arg4277*) in ADGRV1; c.337del, p.(Asp109*) and c.3352del, p.(Gly1118Alafs*7) in CDH23; c.3500G > A, p.(Gly1167Glu) in COL4A3; c.1183C > T, p.(Pro395Ser) and c.1759C > T, p.(Pro587Ser) in COL4A5; c.580+2T > C in EYA4; c.1481dup, p.(Leu495Profs*31) in LARS2; c.1939T > C, p.(Phe647Leu), in MYO6; c.733C > T, p.(Gln245*) in MYO7A and c.242C > G, p.(Ser81*) in TMPRSS3 genes. Here, EYA4 is linked to deafness.