RRHO heatmaps for the ACHE variant, CRTC1 KO, Huntington's disease (HD) R6/1 transgenic, and HTRA2 KO compared with human depression are shown in Fig. 4 While all exhibit matching to down regulated depression genes, CRTC1 also shows robust matching to up genes in depression. This evidence concerns the gene CRTC1 and major depressive disorder.