To determine if the reduction in influenza-specific CD8 T cells was due to reduced numbers of naïve precursors or a result of gaps in TCR repertoire, we adoptively transferred SIINFEKL OVA peptide-specific and MHCI restricted transgenic TCR CD8 T cells from OT-I mice crossed with IL-7Rα449F mice (CD45.2) in to BoyJ mice (CD45.1). Here, CD8A is linked to influenza.