In order to evaluate whether loss of VLA4 would have similar effects on human myeloma cells, Itga4 (α4) CRISPR KO was performed on the human MM.1S cell line, an IgA lambda MM that establishes orthotopic myeloma xenografts when injected i.v. in nod scid gamma (NSG) mice. Here, ITGA4 is linked to plasma cell myeloma.