ITGA4 and plasma cell myeloma: In this study, we generated CRISPR/Cas9-mediated knockout (KO) of the Itga4 (α4) subunit in the VLA4+ 5TGM1-GFP parental murine myeloma cell line (WT 5TGM1) to investigate the biological variation in vitro and in vivo resulting from VLA4 ablation.