In order to experimentally address whether loss of Itga4 prevented KO MM cells from interacting with the stroma, as well as more carefully characterize growth rate, we tested the interaction between BM stromal and the Itga4 KO 5TGM1-GFP cells using the M2-10B4 murine BM stromal cell line, characterized by high fibronectin secretion in vitro40. This evidence concerns the gene ITGA4 and Miyoshi myopathy.