Several genes were identified as significantly mutated genes in GBM namely PTEN, TP53, EGFR, PIK3CA, PIK3R1, NF1, RB1, IDH1, PDGFRA, LZTR1, SPTA1, ATRX, GABRA6, KEL, BRAF V600E, H3.3 histones, EGFR, MET, CDK6), CDK4, MDM2, PDGFRA, SOX2, MYCN, CCND1, CCNE2, PARK2, QKI, TGFbR2, LRP1B, NPAS3, LSAMP, SMYD3, EGFR, CPM, PRIM2, FAM65B, PPM1H, RBM25, HOMER2, EGFRvIII, PDGFRA, p53 pathway (MDM2, MDM4, and TP53), the Rb pathway (CDK4, CDK6, CCND2, CDKN2A/B, and RB1), PI3K pathway (PIK3CA, PIK3R1, PTEN, EGFR, PDGFRA, and NF1), IDH1, ATRX, TERT [8]. The gene discussed is CDK4; the disease is glioblastoma.