To this aim, we targeted three genes [multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), and coiled-coil domain containing 40 (CCDC40)] implicated in the pathogenesis of primary ciliary dyskinesia (PCD), a rare pulmonary genetic disorder, and two genes with therapeutic interest for T-cell engineering [T cell receptor alpha constant (TRAC) and C-X-C chemokine receptor type 4 (CXCR4)]. Here, TRAC is linked to primary ciliary dyskinesia.