We also confirmed in normal (N) and tumor (T) tissues from 8 breast cancer patients that the expression of α-actinin which is a focal adhesion and actin cytoskeleton marker did not change in the presence of Bag-1 expression, even though Akt is still upregulated and phosphorylated in breast cancer (S4I and S4J Fig). This evidence concerns the gene AKT1 and breast cancer.