The impact of TRANK1 overexpression on the morphology of neurons was also investigated via transfection with plasmid Cytomegalovirus DNA (pcDNA3.1(+)) vector encoding human TRANK1. Collectively, this study helps to reveal that gut microbiota may participate in the BD pathogenesis by interacting with a robust BD risk gene. The gene discussed is TRANK1; the disease is Behcet disease.