TMEM158 and central nervous system cancer: We first performed RT-PCR and western blotting to determine the transfection efficiency of lentiviral overexpression (OE-TMEM158) and knockdown (shRNA-TMEM158-1, sh-1; shRNA-TMEM158-2, sh-2) and the expression of TMEM158 in U87MG, U251MG, and TJ905 glioma cell lines (Figs. 3A–B, 4E–F).