Our results showed that (1) miR-30c-5p-EVs could effectively delivered miR-30c-5p to PTC cell, and decrease the mRNA and protein expression of PELI1 and inhibited the PI3K/AKT pathway; (2) treatment with miR-30c-5p-EVs inhibited PTC cell proliferation and migration in vitro; and (3) miR-30c-5p-EVs were efficacious against PTC tumor growth in the BALB/c nude mice model, together with the upregulation of miR-30c-5p and significant downregulation of PELI1, p-AKT, Ki-67, and MMP2 expression. This evidence concerns the gene MMP2 and neoplasm.