A total of 10 fresh P. vivax isolates with > 60% mature trophozoites (median of parasitemia 6800 parasites/μL; IQR: 5440–11,348) were used to test whether PLT activation, specifically the release of PF4/CXCL4, takes place in response to direct interaction with Pv-IEs. This evidence concerns the gene PF4 and parasitic infectious disease.