Furthermore, the KEGG pathway enrichment analysis of tRF-1:28-Val-CAC-2 was enriched in pathways in cancer, which included the Hedgehog signaling pathway with EVI1, BCL2, and PKA (Yip et al., 2006; Lu et al., 2019) and the Wnt signaling pathway (not in the top 10) with Frizzled, Daam1, and NFAT (Ren et al., 2015). This evidence concerns the gene DAAM1 and cancer.