Moreover, we profiled the EMT associated makers in the PS-BC cells, and the Real-Time qPCR analysis results validated that the mRNA levels of the mesenchymal biomarkers, including ZEB1, vimentin, twist, slug, snail and fibronectin, were all elevated by hypoxic stimulations in PS-BC cells (Figures 6I, J). The gene discussed is TWIST1; the disease is breast cancer.