The immunoblotting analyses indicated that nCHI3L1 Abs inhibited AKT phosphorylation induced by rCHI3L1 in various NSCLC, PDAC and CRC cells (Lanes 6-9 vs 2-5, Figure 3H, and Figure S4H), confirming the AKT inhibition function of nCHI3L1 Abs. Here, AKT1 is linked to colorectal carcinoma.