Consistent with previous studies 53, the CCA, CLHCC and classical HCC showed different prognosis (Figure 5B), and the lineage specific markers (EPCAM, CD133, KRT19, CEACAM6, HNF4A and ALB) were also differentially expressed among these three subtypes (Figure 5C). The gene discussed is PROM1; the disease is cholangiocarcinoma.