Many studies have reported the correlation between tumor infiltrating lymphocytes (TILs) and favorable prognosis in different tumor types, people who respond to single-agent PD-1 blockade therapy have a higher density of baseline CD8+ T cells infiltration, we investigated whether single-agent sirolimus had changed the tumor microenvironment by bringing T cells into SMM103 melanoma lesions which responded to anti-PD-1 therapy. The gene discussed is CD8A; the disease is neoplasm.