Secondly, our study describes the previously unknown immunomodulatory effects of Dara in the mitigation of GVHD by the reduced expression of cytotoxic effector molecules (Granzyme A, Granzyme B, Granulysin, Perforin 1), pro-inflammatory cytokines (IL15, IL32, IFNγ, IL6, IL10), chemokines and chemoattractant receptors (CX3CR1, CXCR3, CCR1, CCR2, CCR5, CCL3, CCL4, CCL5) by T cells. Here, PRF1 is linked to graft versus host disease.