To confirm the role of mTORC1 signaling pathway in the CRS-induced depression, mice exposed to CRS for 21 days were divided into four groups, including CRS-vehicle group (CRS-Veh, n = 11), CRS-fluoxetine group (CRS-FLX, n = 11), CRS-rapamycin group (CRS-Rap, n = 9), and CRS-fluoxetine-rapamycin group (CRS-FLX-Rap, n = 9). Here, LRPAP1 is linked to major depressive disorder.