Consistently, MHY1485 caused significant accumulation of neutral lipids and enhanced cellular free fatty acid levels, whereas rapamycin generates opposite effects on cellular lipid content in MCF-7 cells (Fig. 6I and J), collectively indicating the crucial roles of mTOR signaling in SREBP-1 expression and lipid metabolism in breast cancer cells treated with adiponectin. This evidence concerns the gene ADIPOQ and breast cancer.