The samples with CTNNB1 mutations showed up-regulated CTNNB1-HCC class pathway compared with CTNNB1 wild-type tumor samples, suggesting that the discovered CTNNB1 mutations in this cohort induced the activation of WNT/β-catenin pathway (Additional file 4: Fig. S5C and D). Here, CTNNB1 is linked to hepatocellular carcinoma.