THBS1 and parasitic infectious disease: To investigate the significance of TSP1 expression in the molecular colocalization between YAP and β-catenin during the early phase of cellular infection, and how this colocalization affects parasite infection of heart endothelial cells in vitro, we challenged MHEC from WT and TSP1 KO mice with invasive T. cruzi trypomastigotes Tulahuen strain clone MMC20A for different times.