AR and Familial prostate cancer: To verify these findings with the same polyQ tracts that we used for the turbidity assays, we used a luciferase assay in two prostate cancer cell lines (LNCaP and PC3) transfected with vectors expressing full-length AR that contained 12, 20, 31 and 49 glutamines (AR-12Q, -20Q, -31Q and -49Q, respectively).