In the colon, NLRP3 inflammasome was activated and its inflammatory cytokines were released in the mice model of GVHD, while berberine could inhibit the NLRP3 inflammasome (Figure 6A–C), TLR4 signalling (Figure 6D) and suppressed inflammatory cytokines except IL‐6 (Figure 6E,F). The gene discussed is IL6; the disease is graft versus host disease.