Previously Rebbeck et al. [25] described LOH analysis from 14 informative cancers from BRCA1/BRCA2 MINAS cases and found LOH at a single locus in four tumours suggesting that in most cases the tumour develop from a second hit at a single CSG and the effects of MINAS are generally additive rather than synergistic. This evidence concerns the gene BRCA2 and neoplasm.