In addition, RT-qPCR and Western blot analysis suggested that the mRNA and protein expression of DEPDC1B was higher in HCC cell lines SK-Hep-1, HCCLM3 and Huh7 than that in normal hepatic epithelial cells HHL-5 (Figure 1c-d). The gene discussed is DEPDC1B; the disease is hepatocellular carcinoma.