Next, 25 serum samples from healthy individuals (n = 5) and patients with NAFL (n = 5), NASH (n = 5), and NASH-HCC (n = 10) were fractionated by size-exclusion chromatography, and both IgM-bound AIM and IgM-free AIM were measured by ECLIA using beads coated with anti-AIM antibody No. 11 and Ru-labeled anti-AIM antibody No. 8. Here, CD40LG is linked to hepatocellular carcinoma.