Subsequently, 25 serum samples from healthy volunteers (n = 5) and patients with NAFL (n = 5), NASH (n = 5), and NASH-HCC (n = 10) were fractionated in the same manner and assayed for IgM-bound AIM, IgM-free AIM, and IgM by ECLIA. This evidence concerns the gene CD40LG and metabolic dysfunction-associated steatohepatitis.