To evaluate the sample dilution linearity, two serum samples from patients with NASH-HCC (No. 1 and 2) were serially diluted from 1:1 to 1:64 and IgM-free AIM was measured by ECLIA using beads coated with anti-AIM antibody No. 12 and Ru-labeled anti-AIM antibody No. 11. The gene discussed is CD40LG; the disease is hepatocellular carcinoma.