To evaluate the analytical performance of ECLIA, size-exclusion chromatography fractionated samples from the serum of one patient with NASH-HCC were assayed by the ECLIA using magnetic beads coated with anti-AIM antibody No. 11 and Ru-labeled anti-AIM antibody No. 8 to measure IgM-free AIM and IgM-bound AIM. This evidence concerns the gene CD40LG and hepatocellular carcinoma.