The present study demonstrated that H2O2 (ROS accumulation model in the elderly), TNF-α (chronic low dosage inflammation model in the elderly), and high glucose (hyperglycemia model as observed in diabetes) induced HUVECs senescence, up-regulated the expression of miR-335-5p, and down-regulated the expression of SIRT7. This evidence concerns the gene SIRT7 and Hyperglycemia.