Furthermore, to clarify the effect of JMJD4 on kidney cancer, we transfected the pcDNA-3.1(+)-JMJD4 plasmid into Caki-1 cells and verified the overexpression by qRT-PCR and western blot (Figures 8(a) and 8(b)). The gene discussed is JMJD4; the disease is kidney cancer.