By detecting ACSL4 expression in HCC cells with miR-23a-3p mimics or Anti-miR-23a, and 23-KO HCC tumour tissues, it was found that miR-23a-3p negatively regulated the mRNA and protein expression of ACSL4 in HCC cells and tumour tissues (Fig. 6B, C). The gene discussed is ACSL4; the disease is hepatocellular carcinoma.