To further examine if ACSL4 was required for miR-23a-3p-suppressed ferroptosis in sorafenib-treated HCC, we co-transfected miR-23a-3p inhibitor with ACSL4 siRNA to HCC cells in the presence or absence of sorafenib (Fig. S5B, C). This evidence concerns the gene ACSL4 and hepatocellular carcinoma.