qRT-PCR showed that IL-4 and IL-10 levels were significantly increased in the PBMCs of 56 AIS patients (P < 0.05, Fig. 1E and F), similar to the plasma level, but the levels of proinflammatory cytokines (IL-6 and TNF-α) were not changed (P > 0.05, Fig. 1G and H). The gene discussed is TNF; the disease is androgen insensitivity syndrome.