We first assessed the relative expression levels of SCUBE3 in four HCC cell lines, including Bel7404, Bel7402, HepG2, and SMMC7721 and one normal human hepatocyte line HL7702 by real-time PCR (Fig. 1B) and western blotting (Fig. 1C). This evidence concerns the gene SCUBE3 and hepatocellular carcinoma.