ITIH4 and intrahepatic cholangiocarcinoma: P < 0.05 was supposed to be statistically significant, and three of the twenty hub genes were prognostic markers for the disease free survival analysis in ICC patients, but the survival analysis results of AGT and ITIH4 were contradictory to our previous analysis, that was the DEGs analysis results showed that they were downregulated in ICC compared with that in normal bile duct tissue, while the survival analysis showed they were favorable for the ICC patients when they were downregulated.