MYH14 and cyst: Using this protocol, we have also imaged the morphogenesis of septate junctions (SJs) using neuregulin-GFP protein trap transgenic line (Nrg-GFP), which marks the SJs between the cyst cells (Dubey et al., 2019), and the myosin motor localization and dynamics at the actin cap using the GFP-tagged myosin light chain (squash-GFP) transgene expressed under its own promoter (sqh::sqh-GFP) (Kapoor et al., 2021).