RTCA assay results showed that Ctrl-CAR-T alone did not significantly inhibit the tumor cells, but OAV-DEC or CAIX-CAR-T treatment alone could effectively inhibit the growth of renal cancer cells with the extending of incubation time, and the inhibition effect was enhanced after the combination of the two; in addition, the difference was statistically significant compared with the control group in OSRC-2 cells with high CAIX expression. Here, CA9 is linked to neoplasm.