Overall, our study demonstrates that ITGB1 exerts a pivotal role in accelerating cell cycle via paxillin (PXN)/14-3-3 protein zeta (YWHAZ)/protein kinase B (AKT) pathways, which provides a novel insight into the role of ITGB1 in HCC malignant progression. This evidence concerns the gene AKT1 and hepatocellular carcinoma.