Furthermore, our examination on TR GBM unveiled that the upregulated genes unique to the cell types govern signaling pathways such as EGFR, MAPK, phospholipase C-activating G protein-coupled acetylcholine receptor, interleukin-7-mediated, regulation of neural precursor cell proliferation, negative regulation of T cell mediated cytotoxicity and replication. This evidence concerns the gene EGFR and glioblastoma.