Meanwhile, the mRNA and protein expression levels of PAK3 in HCC cell lines (HepG2, Hep3B, Huh7 and HCCLM3 cells) were also examined by qRT-PCR and Western blotting, and the results showed that PAK3 was also highly expressed in HCC cell lines (Figure 2d and 2e). The gene discussed is PAK3; the disease is hepatocellular carcinoma.