For this, HCC cell lines HLE and Huh7 were transiently transfected using specific siRNA pools against PEA15, PPP1CA and TUFT1. Knockdown of PEA15, PPP1CA and TUFT1 decreased cell viability (Fig. 4A) and increased apoptosis (Fig. 4B) in each case, whereby knockdown of PPP1CA showed the strongest induction of apoptosis. Here, PPP1CA is linked to hepatocellular carcinoma.