In order to clarify the correlation between the upregulation of AL135999.1 and METTL3 in CRC, we knocked down the expression of METTL3 in SW480 and HCT116, which resulted in a lower m6A level of AL135999.1 than the control group (Figure S3C-E). The gene discussed is METTL3; the disease is colorectal carcinoma.