Last but not least, the performance of hub genes exerts a great ability in discerning BD and HC (AUC ranging from 0.81-0.88) where CCL4 and NPY2R are proved as a moderate diagnostic biomarker in disease recognition from healthy subjects in our validation cohort (clinical characteristics are provided in the online Supplementary Material, Table S1). Here, CCL4 is linked to Behcet disease.