Furthermore, to assess the immune exhaustion-related gene expression by the different T lymphocyte subpopulations, we used CIBERSORTx, which, when applied to the PanCancer collection data, was able to detect, in the context of CRC microenvironment, the expression of FOXP1 and SIRT1 in CD4+ memory resting and CD8+ cells. This evidence concerns the gene SIRT1 and colorectal carcinoma.