The main signal pathways involved in the treatment of cervical cancer by Hedyotis diffusa were pathways in cancer, hepatitis B and blader C. Pathways in cancer, Hepatitis B, Bladder cancer, Prostate cancer, Pancreatic cancer, TNF signaling pathway, HIF-1 signaling pathway, Non-small cell lung cancer, Chagas disease (American trypanosomiasis), Leishmaniasis and so on. This evidence concerns the gene TNF and American trypanosomiasis.