In osteosarcoma cell lines (MG-63, SAOS-2, and U2OS), TMP inhibited cell proliferation and induced apoptosis and G0/G1 arrest in a dose-dependent manner by upregulating the expressions of cytosolic NF-κB and p65, while downregulating the nuclear expressions of NF-κB, p65, Bcl-2, and cyclin D1. This evidence concerns the gene NFKB1 and osteosarcoma.