TMP could induce colorectal cancer cell line (SW480 and CT26) apoptosis via a p53-dependent mitochondrial pathway and cell cycle arrest at the G0/G1 phase; TMP-induced apoptosis and cell cycle arrest were markedly reversed by pifithrin-α (a p53 inhibitor) (Bian et al., 2021). The gene discussed is TP53; the disease is colorectal cancer.