MMP10 and infection: Active MMP-10 was produced in human PASMCs infected with Adv-MMP-10 (10, 20 MOI) for 48 h or infected with Adv-MMP-10 (10 MOI) for 48 and 72 h, as determined by immunoblotting of conditioned medium from serum-starved cells (Fig. 4A and B), indicating that the active MMP-10 was significantly upregulated by Adv-MMP-10 infection in human PASMCs.