As increased cell migration was an important aspect in metastasis and positively correlated with the degree of malignancy and the mortality of ccRCC patients, wound healing assay and transwell analysis were performed using 786-O/ACHN-G6PDOE, 786-O/Caki-1-G6PDSi and relevant control cells to evaluate whether G6PD imparted the migration ability of ccRCC cells. The gene discussed is G6PD; the disease is nonpapillary renal cell carcinoma.